Antibody Phage Display: Technique and Applications
نویسندگان
چکیده
منابع مشابه
Antibody phage display: technique and applications.
inTrOducTiOn The production of human monoclonal mAbs for research and clinical use is closely related to the development of phage display technology, initially described by Smith in 1985 and further developed by other groups (e.g., Winter, McCafferty, Lerner, Barbas). Antibody phage display (APD) is based on genetic engineering of bacteriophages (viruses that infect bacteria) and repeated round...
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Antibodies are an essential biotechnological tool being a part of immunodiagnostics, therapeutics and life science research instruments. Due to the advances of molecular biology methods, it became possible to manipulate structure and properties of antigen-binding domains of antibodies (1). This led to the creation of a variety of small-sized, single-domain, bi-specific, conjugated antibodies, a...
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Since the advent of phage display technology, dating back to 1985, antibody libraries displayed on filamentous phage surfaces have been used to identify specific binders for many different purposes, including the recognition of tumors. Phage display represents a high-throughput technique for screening billions of random fusion antibodies against virtually any target on the surface or inside can...
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ژورنال
عنوان ژورنال: Journal of Investigative Dermatology
سال: 2014
ISSN: 0022-202X
DOI: 10.1038/jid.2013.521